Company: Hengshui Shanzhi Health Drink CO.,LTD
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The influence of the foundation blood sugar value of the Young Buckwheat Leaves Powder to the streptozotocin causing blood sugar of the small rat goes up.
Purpose
The streptozotocin can hurt insular B-cell, causing the blood sugar to increase high, form the diabetes model. This experiment observation is subjected to the reagent to the influence of the foundation blood sugar value of the Young Buckwheat Leaves Powder to the streptozotocin causing blood sugar of the small rat goes up.
The material of experiment
1.Medicine:
Be subjected to the reagent : the Young Buckwheat Leaves Powder, which provided by Hengshui Shanzhi Health Drink CO., LTD, dark green powder, the dosage is 3--6 gs/ person/ day, in the light of 60 kg weight calculation, is 0.05g /kg. The small rat is subjected to try the amount of: 1g , 0.5g , 0.25g / kg.
The masculine gender checks against the medicine : Metformin Hydrochloride Tablet, LT0412062 , the product Sino-American Shanghai Squibb Pharmaceuticals Ltd , batch number:LT0412062, the small rat is subjected to try the amount of: 0.3g /kg.
Building the high blood sugar model medicine : Streptozotocin, buy from America Sigma CO.,LTD, the dose of building the mold is 150 mg/ kg.
2.Animal:
Small rat of ICR with the weight of 18- -20 g , half is female, half is male, providing by Beijing Vital River Laboratorium Animalis Ltd, qualified certificate number: 2002--2003 of SCXK(Beijing)
3.Instrument:
Ultra Blood Glucose Monitoring System : specification model number:010-635-01, the product of Life Scan International Inc
One Touch Ultra Test Strips : the product of Life Scan International Inc.
The method and result
1.Dividing the groups with medicine:
The small rat is divided into six groups: comparison group, model group, Metformin Hydrochloride Tablet 0.3g /kg/group , the Young Buckwheat Leaves Powder is 1g , 0.5g , 0.25g /kg/group. Each group starts to infuse the medicine to stomach after seventh day of building the mold, giving medicine 14 times, everyday once. The comparison group infuses with same quantity drinking water to the stomach.
2.The method of building the high blood sugar model:
In addition to comparison group, each group small rat forbids to eat after 12 hours, the belly cavity injects the plain 150 mg/ kg of the streptozotocin, returning of feeding in cage.
3.Method:
After 30 minutes of each small rat giving medicine at the end time, behind stopping the medicine four, nine days(16 hours of forbidding the food and water), the tail point puts out minim blood, placing it on the One Touch Ultra Test Strips easure the empty stomach blood sugar with the blood sugar instrument. Compute the average blood sugar value of each group, the criterion value, compared with the comparison group, inspect by t.
4.Result:
The result as following table 1:
The table 1 shows that the influence of Young Buckwheat Leaves Powder to the streptozotocin with the result that the blood sugar goes up the small rat foundation blood sugar value (Unit: mmol/L)
group |
dose ( g/kg ) |
animal quantity |
the blood sugar after 14 days dose |
the blood sugar after 4 days no dose |
the blood sugar after 9 days no dose |
modle group |
- |
10 |
6.38±0.78 |
18.93±4.16 |
16.79±5.74 |
Young Buckwheat Leaves Powder group |
1 |
10 |
5.38±0.85* |
10.97±4.50** |
14.17±4.53 |
|
0.5 |
10 |
6.28±1.20 |
11.78±2.80** |
12.67±3.22 |
|
0.25 |
10 |
6.62±1.28 |
16.28±6.34 |
21.85±7.70 |
Metformin Hydrochloride Tablet |
0.3 |
10 |
5.34±13.10** |
17.94±4.83 |
22.28±6.14 |
comparison group |
- |
16 |
5.11±0.60** |
6.13±0.63** |
5.32±0.65** |
Annotation: compared with the modle group **P<0.01 , *P<0.05
The results show that: The streptozotocin can hurt the insular B-cell ,causing the blood sugar to increase high, form diabetes model. The small rat foundation blood sugar value is obvious higher than the comparison group. After the medicine infusing to the stomach with the Young Buckwheat Leaves Powder 1 or 0.5g /kg 14 days no dose, the foundation blood sugar value of small rat is lower than the model group. Among them, the Young Buckwheat Leaves Powder with function 1g / kg is most obvious. ( compare with the model group P<0.05), the foundation blood sugar value of small rat is lower than the model group(compare with the model group P<0.01). After the medicine infusing to the stomach with the Young Buckwheat Leaves Powder 1 or 0.5g /kg 9 days no dose, the foundation blood sugar value of small rat is also lower than the model group, there is no obvious difference of statistics.